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General Reagents 5U/uL T4 DNA Ligase
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | E1014-A |
Payment & Shipping Terms:
| Minimum Order Quantity: | 200 U |
|---|---|
| Price: | USD 18.44-47.5/200 U |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Product Name: | T4 DNA Ligase | Cat. No./Spec.: | E1014-A/200 Weiss U; E1014-B/1,000 Weiss U; E1014-C/5,000 Weiss U |
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| Appearance: | Colourless | Application: | DNA Ligation |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | 5U/uL T4 DNA Ligase,General Reagents 5U/uL T4 DNA Ligase |
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Product Description
T4 DNA Ligase
Cat. No./Spec.:
E1014-A/200 Weiss U; E1014-B/1,000 Weiss U; E1014-C/5,000 Weiss U
Concentration: 5 Weiss U/µL
Product Description
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA or RNA. This enzyme is capable of repairing single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrids, and can join DNA fragments with sticky or blunt ends, but it is inactive on single-stranded nucleic acids.
T4 DNA Ligase requires ATP as a cofactor.
Storage Condition & Shelf Life
Store at -20°C. The product is valid for 2 years.
Source
Recombinant E. coli strain containing the gene 30 cloned from bacteriophage T4.
Unit Definition
At 37°C, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [32PPi] into an activated carbon adsorption state within 20 minutes.
Enzyme activity is determined in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 μM [32P]orthophosphate (PPi).
One Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16°C. λ DNA fragments.
Scope of Application
- DNA fragments generated by cloning restriction enzymes
- Cloned PCR products
- Ligation of double-stranded oligonucleotides or adapters to DNA
- Site-directed mutagenesis
- Amplified fragment length polymorphism (AFLP)
- Ligase-mediated RNA detection
- Nick repair in double-stranded DNA, RNA, or DNA/RNA hybrids
- Self-circularization of linear DNA
