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RNase H Molecular Tool Enzyme
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | E1016 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 100 U |
|---|---|
| Price: | USD 22-29/100 U |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Product Name: | RNase H | Cat. No./Spec.: | E1016-A/100 U, E1016-B/500 U |
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| Appearance: | Colourless | Application: | Removal Of MRNA |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | RNase H,RNase H Molecular Tool Enzyme |
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Product Description
RNase H
Cat. No./Spec.: E1016-A/100 U, E1016-B/500 U
Concentration: 5 U/µL
Product Description
Ribonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.
Cat. No./Spec.: E1016-A/100 U, E1016-B/500 U
Concentration: 5 U/µL
Product Description
Ribonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.
Storage Condition & Shelf Life
Store at -20°C.
Source
E. coli strain MRE-600.
Unit Definition
A unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.
Enzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl2,1 mM DTT, 24 µM [3H]-poly(A)·poly(dT), 0.03 mg/mL BSA, and 4% (v/v) glycerol.
Scope of Application
- Removal of mRNA before the synthesis of the second-strand cDNA
- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis
- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)
- Site-specific cleavage of RNA
- Study of products from in vitro polyadenylation reactions
Store at -20°C.
Source
E. coli strain MRE-600.
Unit Definition
A unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.
Enzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl2,1 mM DTT, 24 µM [3H]-poly(A)·poly(dT), 0.03 mg/mL BSA, and 4% (v/v) glycerol.
Scope of Application
- Removal of mRNA before the synthesis of the second-strand cDNA
- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis
- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)
- Site-specific cleavage of RNA
- Study of products from in vitro polyadenylation reactions
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